Do you need to use a surface marker? How about using one of the membrane stable PKH dyes sold by Sigma? These are incredibly bright, don't "bleed" from one cell to the next and are functionally inert. You would just incubate your Jurkats in the dye prior to your adhesion assay. Calman ---------- From: kjartan.egeberg@medisin.ntnu.no Sent: Wednesday, June 4, 1997 3:46 AM To: cyto-inbox Subject: Jurkat marker Hi flowers. Yesterday I sent a request for a good surface-marker to stain Jurkat cells. Rob McGilp wrote that CD95 is well expressed on these cells. Thank you for your reply. It is true that Jurkat express CD95, but since Fas/Fas-L is part of the study (adhetion of apoptotic cells), I have to use another marker to follow the Jurkats. I intend to use cell tracing. But I need a surface-marker to separate those adhered to targets from those that are been phagocytosed. I'm still on my tip-toes with hopes for a good surface-marker. Thanks. Kjartan Egeberg Inst. of Canser Research and Mol. Biol. NTNU, Trondheim, Norway E.mail: kjartan.egeberg@medisin.ntnu.no
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:49 EST