KJ, in our hands, CD5 seems to be a good surface marker for Jurkat cells. We use it together with log FSC to discriminate between normal and apoptotic T cells, CLL B cells and Jurkat cells in short-term mixed cultures; Jurkats are the larger CD5+ cluster. Our 4-color permutations include CD3, CD5, CD19, CD95, AnnexinV, 7AAD; we did not encounter any difficulty with these combinations. Good luck! From: kjartan.egeberg@medisin.ntnu.no Date: Wed, 4 Jun 1997 09:46:35 +0200 (METDST) To: cyto-inbox Subject: Jurkat marker Hi flowers. Yesterday I sent a request for a good surface-marker to stain Jurkat cells. Rob McGilp wrote that CD95 is well expressed on these cells. Thank you for your reply. It is true that Jurkat express CD95, but since Fas/Fas-L is part of the study (adhetion of apoptotic cells), I have to use another marker to follow the Jurkats. I intend to use cell tracing. But I need a surface-marker to separate those adhered to targets from those that are been phagocytosed. I'm still on my tip-toes with hopes for a good surface-marker. Thanks. Kjartan Egeberg Inst. of Canser Research and Mol. Biol. NTNU, Trondheim, Norway E.mail: kjartan.egeberg@medisin.ntnu.no
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