Hi, everyone: I have an investigator who wants to look at intracellular distribution of a drug using confocal laser scanning microscopy. The drug is not fluorescent, so he has a monoclonal antibody (FITC conjugate) to the drug. Our question is, during permeablization to allow the antibody into the cells, what is the best way to prevent the drug from leaching out of the cells? I suppose a pre-permeablization paraformaldehyde fixation step would be helpful, but I would like to hear from anyone who has done such drug studies and knows if this is an effective approach. Thanks in advance.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:47 EST