Last week I sent a message asking for feedback on how people dealt with the CAP checklist since it required isotypes for cd4 testing. Thanks to all that replied to my query! I was going to post a summary, BUT - the checklist has changed. Many thanks to Karen Munn at Duke for bringing this to my attention!! And - Thanks to CAP for improving their checklist. 1997.0 Edition under Blood Lymphocyte Subset Enumeration states: "Is there a procedure to set markers (cursors) to distinguish fluorescence-negative and fluorescence-positive cell populations?" 1997.0 Edition under Leukemia and Lymphoma states: "Is there a procedure to distinguish fluorescence negative and fluorescence-positive cell populations?" "NOTE: This does not imply that a separate negative control sample must be run. It is possible to coordinate panels of monoclonal antibodies to compare the binding of monoclonal antibodies of the same subclass that typically have mutually exclusive patterns of reactivity of subsets of hematopoietic cells. In this way, test antibodies may also double as control reagents."
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:44 EST