Dear All, We recently embarked on an alveolar macrophage project, staining for surface molecules. (Fun, fun, fun) As perviously suggested by the kind members here, the autofluorescence of these cells is somewhat of a pain. However I found some papers that could help others who face similar problems in overcoming them. One paper actually uses the autofluorescence to an advantage. "Multiparameter flow cytometric analysis of inflammatory cells contained in bronchoalveolar lavage fluid. J.Imm.Meth. 172(1994) 59-70" "Application of a flow cytometric method using autofluorescence and tandem fluorescent dye to analyse human alveolar macrophage surface marker. J.Imm.Meth. 172(1994) 17-24" We are using this tandem dye (R-PE-Cy5 - excitation 488nm emission 660+-20nm). Has anyone come across flow standard beads that will enable us to quantitate fluorescence (MESF) at this emission wavelength. I have looked in the FCSC catalog but no banana. Fanx in advance Geza Paukovics - Flow Laboratory - AIDS Pathogenesis Research Unit Macfarlane Burnet Centre for Medical Research .***. .***. .** PO Box 254 _--_|\ * | | | * * | | Fairfield, VIC 3078 / \ * * | | | * * | | | AUSTRALIA. \_.--._/ * * | | | * | | | * ph. (+61 3) 9282 2132, O '***' '***' FAX (+61 3) 9282 2100
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