---------- Forwarded message ---------- Date: Wed, 23 Apr 1997 10:44:55 -0400 From: Stacie Anderson <staciea@nhgri.nih.gov> To: cyto-inbox Subject: Re: Intracellular cytokines in monocytes We use CD14 as our monocytic marker and it does not seem to be affected by the stimulation although we have not specifically stained the cells pre and post stimulation. We had a monocyte product from apheresis and the staining was as we would have expected. We did see a difference in IL-12 production depending on the stimulation that the cells received. Good luck! Stacie Stacie Anderson Laboratory of Gene Transfer, Hematopoeisis Section NCHGR, NIH Room 2C12 49 Convent Drive Bethesda, MD 20892-4442
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