--- On Mon, 21 Apr 1997 19:03:25 GMT Dr James Clark <JCLARK@clinmed.gla.ac.uk> wrote: >I have recently been attempting Annexin V staining and so far this >has been succesful. However I am worried about the amount of debris in the >bottom left of FSC/SSC, should this be included in the final analysis >or excluded? > I guess the answer depends on whether the events w/ low FS ansd SS are apoptotic cells or debris or some other cell type [depending on the system]. For th e most part, the info you want is % apoptotic cells of a particular population. So you need some method to determine whole cells [apoptotic or live] from other events. If you can't achieve this with LS then maybe adding a McAb or staining for DNA wil l be a required gating step. Good luck, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] 4/23/97 11:28:52 AM E-mail: thomasm@nshs.edu --------------------------------------------------------
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