--- On Tue, 22 Apr 1997 08:54:03 -0600 Anna Travelstead <travelst@wpsmtp.siumed.edu> wrote: The PI wants >DNA cell cycle analysis, proliferation index and apoptosis index >performed. Does anyone have recommendations on the best apoptosis >kit for use on a FACS Vantage? The easiest way to measure these 3 parameters is to stain for DNA content which allows you to quantitate apoptosis [below diploid] and proliferation [above diploid] simultaneously. If you wish to analyze cell death using a different method you have many choices, including TUNEL, annexin, which are in kit form. There are also many other methods discussed in current literature. Good luck, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] 4/23/97 11:37:08 AM E-mail: thomasm@nshs.edu --------------------------------------------------------
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