Jane, Contact the good people at Phoenix Flow Systems. They sell this as a kit which includes positive and negative controls. They can be reached at 800-886-3569 or email: info@phnxflow.com. Lisa Adams Cancer Research Pharmacia & Upjohn Kalamazoo, MI ______________________________ Reply Separator _________________________________ Subject: apoptosis Author: "Hughes; EJ; Jane; Ms" <JHUGHES@ICH.UCT.AC.ZA> at INTERNET Date: 4/22/97 11:30 AM Dear Flowers We are setting up an apoptosis assay using FITC anti BrdU ( the method of Xun Li, Traganos and Darzynkiewicz) and I have a few questions (I do hope not TOO basic!!) 1. Should one use an Isotypic control. 2.I want to immunophenotype first, should I lyse the RBC after immunophenotyping and before DNA cleavage. (I wasn't sure from the method whether Triton would lyse the cells as RBC lysis is not given as a step) 3. What are the options for positive controls. Thanks Jane Hughes Immunology Section ICH Lab, Dept Paediatrics Red Cross Children's Hospital Rondebosch, Cape Town, South Africa
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:41 EST