If I have a mixture of 3 unlabeled mouse monoclonal Abs to different cytokeratins, how do I choose an isotype control concentration? Each individual Mab is at 1 microgram/ml (total of 3 micrograms/ml) and each is an IgG1 isotype. Would you suggest I use a single unlabeled IgG1 control at 1 or at 3 micrograms/ml? I also want to use these for some immunohistochemistry staining. Can I use the same isotype control for this? Thanks for any comments! Tim Holzer Centocor, Inc. holzert@centocor.com 610-889-4492
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