apoptosis

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Dear Flowers

We are setting up an apoptosis assay using FITC anti BrdU ( the 
method of Xun Li, Traganos and Darzynkiewicz) and I have a few 
questions (I do hope not TOO basic!!)

1. Should one use an Isotypic control. 
2.I want to immunophenotype first, should I lyse the RBC after 
immunophenotyping and before DNA cleavage. (I wasn't sure from the 
method whether Triton would lyse the cells as RBC lysis is not given 
as a step)
3. What are the options for positive controls.

Thanks

Jane Hughes
Immunology Section
ICH Lab, Dept Paediatrics
Red Cross Children's Hospital
Rondebosch, Cape Town, South Africa

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