Dear Flowers I am setting up an assay to screen for the expression of CD18, 11a,11b and 11c on neutrophils; taking mean peak intensity of resting versus stimulated cells. Is it possible that some of you have used Ca ionophore for this purpose and could advise me as to an appropriate concentration fro stimulation ( or advise a reference). Many thanks Jane Hughes Immunology Section Dept Paediatrics Red Cross Children's Hospital Rondebosch, Cape Town, South Africa
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