I have actually seen a similar case. It was also a T-cell ALL with a similar phenotype. However, the antibody you use for the TdT is very important - not all of them will recognize all epitopes. We discovered that the mixture of three different clones from SuperTechs worked the best. We had actually frozen down quite a lot of the cells from that patient (he had a very high WBC) to use for antibody controls and cross-checks, and they didn't work with one of the single-clone TdT antibodies we tried. All the other phenotypes stayed true to form, though. So be careful about your antibody choice! Karen Tamul FAST Systems, Inc Gaithersburg, MD
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