We recently ran phenotyping on bone marrow cells from a 21-year old patient having cells which appeared morphologically to be lymphoblasts. The cells were CD2+, CD3+, CD7+, CD45+, DR-, CD5- and Tdt-. This is the first case we have seen of Tdt negative lymphoblasts and were wondering how often this has been observed by others. Our cases of pediatric ALLs are usually CD45- and Tdt+, suggesting that our current case may have a slightly more mature phenotype although presenting clinically as an ALL. A second unrelated question is how to achieve maximal viable cell recovery on anaplastic large cell lymphomas. Although we usually recover sufficient numbers of viable lymphoid cells on fresh lymph node biopsies, we occasionally will have a case in which the cell recovery is extremely low even though the original tissue sample appears adequate. We routinely gently mince the tissues on a fine mesh screen and wash the cells with HBSS before staining. Any contaminating red cells are lysed with ammonium chloride before staining. Have others run into problems with lymphoma cells that are extremely fragile? Thank you in advance for your suggestions. Lucy Kimura Department of Pathology Tripler Army Medical Center TAMC, Hawaii 96859-5000 Lucille_H.Kimura@TAMC.CHCS.AMEDD.ARMY.MIL
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:39 EST