Flowers! Does anyone have any experience with sorting bacteria (Streptococcus) on a stream in air flow cytometer. I am using a FACSTAR Plus. What I think I need is a dye that will allow me to counter stain all the bacteria (preferably live) to be used in conjunction with a dim surface label, and then sort on the surface labeled positives. The counter stain would need to be dim so as not to interfere with the dim surface label. I have tried to sort with a SSC log threshold. and this does not work. As soon as I vibrate the stream I loose all low end resolution which is where my bacteria are. I need to use a fluorescent trigger to threshold on, this would be less sensitive to stream vibrations which is what I assume is killing me on the log SSC threshold. Just a note all my Scatter parameters are on logarithmic amplification. Thanks in advance for your time and energy Philip Barren Pb
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