--- On Sun, 30 Mar 1997 23:27:07 -0500 (EST) MORGANTL@AOL.COM wrote: >We are exploring apoptosis induced in B cell lines by antineoplastic drugs. > We suspect that induction of FAS ligand may play a role with one or more >drugs. Antibodies to FAS-L exist and an Fc fragment that inhibits binding of >FAS-L to the FAS receptor is also now available. My question is this: is it >enough to establish the FAS link by demonstrating a reduction in apoptosis >with either of these agents (the anti-FASL antibody or the Fc fragment) or is >it necessary to use both? I would suggest the following course of experiments: -determine whether the cell line in question expresses FasR and what happens to the levels upon drug treatment -attempt to induce apoptosis in the cells with an inducing anti Fas Ab or souluble Fas L in th e presence or absence of drug -then attempt to block this interaction with either or both of the reagent s you mentioned -remember that , depending on the specific system,modulatin of many other molecules [both pro and anti apoptotic] may be involved in inducing or preventing cell death Good luck, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] 4/1/97 12:45:41 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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