To-Pro-3 Iodide

From: carolyn.surdyk@chmcc.org
Date: Tue Apr 01 1997 - 12:02:39 EST

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I am currently using To-Pro-3 Iodide from Molecular Probes
as my DNA counterstain for confocal microscopy on cytospun,
paraformaldehyde fixed thymocytes.
Even at 0.05 uM (1:20000 dilution from 1mM stock)
I still get interference between the To-Pro and a FITC
labelled secondary antibody to a nuclear protein.
Any suggestions?  I can not use DAPI or PI as my counterstain
either.

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