Hi to all fellow Calibur users out there, HELP!!! In October we received a FACSCalibur with a loader, powermac, and no second laser or sorting option. Since then we have been trying to get the Calibur with the loader to run, without much success. We also have the BDPAC connection problems so many others have, but we can work around that if we only have to start the machine once a day. However, we are experiencing another problem we have not found a way around. When we open the cover to reseat a tube or replace a cracked tube, or sometime even to change to the next rack, we often get an "invalid tube sequence error". At that point we are forced to stop the run entirely. If we simply restart the run again without changing a thing, the run will continue just fine. Anyone running this system knows how much time it takes to generate a report and relaunch the software. It clearly is not a walk-away from system. So far we have replaced the loader, the electronics module on the loader, the entire computer, disconnected our system from the network, reloaded the software multiple times, rebuilt the desktop daily, run Norton utilities, loaded the newest version of CellQuest 3.0.1f, and taken up drinking....and still get the intermittent "invalid tube sequence error". Any suggestions would be of GREAT help. We are at the end of our rope. Thanks in advance, Holly Holly B. Lamb Flow Cytometry /Optical Morphology Core Facility Mayo Foundation Rochester, MN 55905 lamb.holly@mayo.edu 127-5198, (507) 284-4241
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