conjugation vs. sandwich

From: Dave Novo (novod@muss.CIS.McMaster.CA)
Date: Thu Mar 27 1997 - 00:06:24 EST


Hello everyone,

	I have to buy an antibody for which it seems that there is no 
company selling one pre-conjugated to a dye. I was wondering what people 
thought of the relative merits of 
conjugating FITC to the antibody myself   vs.
buying a generic anti IgG-FITC premade conjugate and using a sandwich 
type technique. 

What are the pro's and cons of the various ideas?

Thanks in advance,
Dave

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Deep thought of the day:
If the local 7-11 is open 24 hours a day - why are there locks on the 
doors?
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