Hi All, As Matthias has noted you can simply copy-paste CellQuest elements into PICT accepting programs (this can be very tedious if you have limited memory and 'hungry' programs like Canvas). However this does not get around the inherent problem of poor image quality from CellQuest. If you compare a histogram from CellQuest with a histogram from, say, WinMDI, you will know what I mean. Looking through J.Ex Med recently it was painfully obvious which papers had used CellQuest to generate their figures. We have been trying to find away around this problem for a while and the 'best' solution we have come with so far is as follows:- - Construct your histogram/dotplot etc in CellQuest several times bigger than you intend your final figure to be - Copy and paste to a drawing program (I have only tried Canvas 3.5). In Canvas you then need to convert the entire pasted group to a bit-mapped object - Cut out only the parts inside the frame and delete the rest before pasting back (ie get rid of the border and labels) - Scale your very large plot down to a size to fit into an appropriately sized plot frame with labels that you have previously contructed within Canvas. The down-scaling increases the image quality you get (ie the lines in histograms are much smoother) - If you wish you can then increase the resolution of internal bit-mapped part (you need to experiment here - high resolution gives nicer lines but they can become too fine, especially if you want to make slides or overheads)... After setting it up initially you can save templates in Canvas and Cell Quest that you use again. I would only recommend trying this on machines with a decent amount of RAM (I tried to do it on a 7600 with only 16MB and nearly drove myself crazy waiting for virtual memory). Obviously this is an extremely tedious procedure but it does produce much better histograms than a simple cut and paste. It is far from perfect and we would welcome any suggestions!! We have almost come to the point where we are considering using another program (eg WinMDI) to produce publication quality images... Hope this helps. Adrian ****************************************************** Adrian Smith (PhD Student) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: 61-2-9565-6197 Fax: 61-2-9565-6105 ******************************************************
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