Anti-FAK and Anti-VIN

From: Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Date: Tue Mar 25 1997 - 13:40:24 EST

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Hi everyone,

We are trying to label CHO cells in suspension with both anti-FAK (Focal 
Adhesion Kinase) and anti-VIN (vinculin).  We get great labelling with 
the cells grown on coversips for confocal, but nothing when they are in 
suspension.  Has anyone done either of these on cells in suspension, or 
do the receptors disappear when the adherent cells are removed from their 
substrate???

Thanks in advance.

Deb Berglund
Montana State University

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