Pete Macardle asked about monoclonal antibodies by injecting the mouse with a band of protein cut from an acrylamide gel. We used a method published in: Bennet, R. et al. The production and characterization of murine monoclonal antibodies to a DNA receptor on human leukocytes, J. Immunology 140: 2937-2942, 1988. I don't believe that polymerized acrylamide is toxic, but in this procedure, we eluted the protein and removed the bits of acrylamide by centrifuging. Best wishes, Tony Bakke
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