I was going to reply "privately" but feel that others may want to comment on my comments ! It seems strange to me that the talk about MPO "background staining" is being looked at as simply that. Most other commercial and in house methods are actually INDUCING the activity by releasing the enzyme from Neutrophil lineage cells which then gets drawn into the target population. No amount of blocking will help in this case. How An der Grub "prevent" this I do not know but it may have something to do with the tremendous amount of "autofluorescence" seen after permeablisation compared to, say, a Triton method. Maybe a sort of covering-up effect. Nevertheless, it does seem to work consistently with MPO. As for TdT - I would argue the point that it doesn't work at all but I would be the first to agree that you can get better results with harsher permeablisation methods. Just in case anyone thinks I'm "having a go" at Dako - we use their excellent anti MPO and the HT6 TdT clone marketed by them (? Supertech in the states). Wal Sharp SQU Oman.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:34 EST