Dear Elaine In common with many clinical flow'ers we routinely use Caltag Fix and Perm reagent for MPO detection. It is true that most "in house" methods of permeabilisation will work for MPO. We have tried various methods using non-ionic detergents and commercial rbc lysing solutions. They all had a common fault when using whole blood or bone marrow, positive MPO expression is "induced" in otherwise negative cells by effusion from MPO positive myeloid cells. Somehow the Fix & Perm reagents overcome this problem. If you are using relatively pure cell suspension i.e. less than 5% contaminating normal myeloid cells then most permeabilisation methods will work, if not I recommend Fix & Perm. Despite what Ellen Ko said, Fix & Perm works well for the detction of TdT particularly if you use the HT6 clone, which is available from Supertech and DAKO. Dr Elisabeth Paietta has published many papers on the subject. Regards ********************************************************************* Len Brown 102554.2147@compuserve.com Department of Haematology (968)515736 voice Sultan Qaboos University (968)513419 fax Al Khod, Code 123, Sultanate of Oman
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:34 EST