acrylamide and monoclonal antibodies

From: Pete Macardle (Peter.Macardle@cc.flinders.edu.au)
Date: Fri Mar 21 1997 - 01:30:55 EST 

Dear all,
I remember seeing a technique for making monoclonal antibodies by injecting
the mouse with a band of protein cut from an acrylamide gel. I cannot find
the reference on Medline. Is anyone familiar with this method? In particular
I want to know how much acrylamide a mouse can have before toxic effects
overcome it.

Thanks in advance.
Pete

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