Good morning ladies and gentlemen, I recently found a lot of Actinomycin D in my lab (sweet memory of the Former SU). I was somewhat disappointed when realized that unlike 7AAD it cannot be used for DNA staining. What a pity! I have several grams of that stuff. Can anybody tell me whether I can use my Actinomycin in some way, in relation to flow? May I use it, e.g., to block Pyronin Y binding to DNA for measurement of RNA specific fluorescence? Or is it possible to make 7AAD from Actinomycin using relatively inexpensive chemicals? Does it worth of doing? I suspect that home-made dye may contain too much unmodified Actinomycin, which may reduce DNA staining competing with 7AAD for binding. Did anybody in the panel face this problem before? NB. Please, don't send suggestions like or "you may use PI for DNA staining" (I am not entirely stupid). "you may purchase 7AAD from Molecular Probes, Cat.#00000" (alas, I may not), Many thanks in advance! Vadim Chromyak, Research Center for Radiology, Kiev, Ukraine. PS. I want to thank cordially Nancy Perlmutter and David Coder for sending me protocols and reprints regarding my question about "DNA plus surface staining".
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