To all flow colleges, I have been asked by one of our pathologists to look into the detection of platelet microparticles using flow cytometry (with CD42b) as a diagnostic test for heparin-induced thrombocytopenia. He gave me an article by David Lee et al: British Journal of Haematology, 1996, 95, 724-731 which includes a procedure. However, as we all know, there is no such thing as a straight-forward Flow procedure. So, my questions are: 1. has anybody had experience with this procedure/test and is willing to share it? 2. is this a relatively easy procedure which can be incorporated into a clinical lab (we do mostly DNA, CD4's, leukemia/lymphomas, and retics) without spending too much time on getting the procedure "fine-tuned"? 3. what are the pitfalls? 4. Is the flow procedure widely accepted? 5. how is one reimbursed? Thank you very much in advance for your responses Andrea Illingworth Dahl-Chase Diagnostic Services/Flow Cytometry 333 State Street Bangor, Maine 04401 (207)990-4855
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