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From: "William Davis" <davisw@vetmed.wsu.edu>
Organization: WSU College of Veterinary Medicine
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Date: Thu, 6 Mar 1997 06:42:50 PST8PDT
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Subject: Identification of monocyte/macrophages
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This is a reply to the query about the identification of monocytes and
macrophages cross species. The responses thus far have been indirect except
for identification by side and forward light scatter. Ongoing investigations
have shown that there are many anti-CD14 MoAbs that recognize highly conserved
determinants. Examples that I am familiar with are: DAKO is TUK4, Coulter My4,
and VMRD Inc. CAM36A (a MoAb we developed). Our comparative studies have shown
CAM36A reacts with bovine, caprine, ovine, porcine, feline, canine, llama,
alpaca, ferret, and rabbit CD14. The level of expression is high on
monocytes/macrophages. The level of expression is variable in different
species. We have not conducted extensive studies with the other two MoAbs but
they do react with CD14 in multiple species. None that we have tested,
however, react with equine monocytes/macrophages.
VMRD, Inc.
http://www.vmrd.com/
vscott@vmrd.com
vtech@vmrd.com
vorder@vmrd.com
William (Bill) C. Davis, Ph.D.
WorldWideWeb: http://www.wsu.edu:8080/~davisw
e-mail: davisw@vetmed.wsu.edu
FAX:1-509-335-8328
Phone: 1-509-335-6051
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