Dave I will refer you to our paper. Marie D, Partensky F, Jacquet S, Vaulot D (1997) Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl Environ Microbiol 63:186-193 In fact we calibrate the flow rate of the FACScan and then record the time of each analysis. We have been using this method for several years and it is very very accurate (a few %) provided the flow rate calibration is done often enough (2-3 times per day). Daniel Vaulot ______________________________________ Daniel VAULOT Station Biologique CNRS - INSU - UPMC BP 74 29682 Roscoff Cx FRANCE Ph: 33 2 98 29 23 34 Fax: 33 2 98 29 23 24 e-mail: vaulot@sb-roscoff.fr W3: http://www.sb-roscoff.fr (PhytoPlankton Group)
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:27 EST