Mail*LinkŪ SMTP GFP Hi: I was curious if people had been successfully analysed GFP levels within transfected cells using flow? I once had an investigator try something like this (though I do *not* know if it was GFP, per se) and the experiment was a complete bust! There was something about the excitation/emmision that was not near instantaneous and there was enough delay that no fluoresence could be detected except when using a flourescent microscope. David ------------------ RFC822 Header Follows ------------------ Received: by amgen.com with ADMIN;25 Feb 1997 12:53:12 -0800 Received: from castle-smtp.amgen.com (castle-smtp [138.133.19.51]) by www-int.amgen.com (8.8.5/8.8.5) with SMTP id MAA29477; Tue, 25 Feb 1997 12:39:16 -0800 (PST) Received: by castle-smtp.amgen.com; id MAA23653; Tue, 25 Feb 1997 12:39:15 -0800 Received: from flowcyt.cyto.purdue.edu(128.210.60.31) by castle-smtp.amgen.com via smap (V3.1) id xma023622; Tue, 25 Feb 97 12:38:43 -0800 Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyto-sendout id PAA27907; Tue, 25 Feb 1997 15:08:58 -0500 Received: from imm2 by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6.9/930416.SGI.AUTO) for <cytometry@flowcyt.cyto.purdue.edu> id LAA26123; Tue, 25 Feb 1997 11:35:03 -0500 Received: from imm18 by imm2 (SMI-8.6/SMI-SVR4) id KAA14472; Tue, 25 Feb 1997 10:24:50 -0600 Message-Id: <3.0.32.19970225102937.0070ea04@imm2.imm.uth.tmc.edu> X-Sender: dhavilan@imm2.imm.uth.tmc.edu X-Mailer: Windows Eudora Pro Version 3.0 (32) Date: Tue, 25 Feb 1997 10:29:37 -0600 To: cyto-inbox From: "David L. Haviland, Ph.D." <dhavilan@imm2.imm.uth.tmc.edu> Subject: GFP Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
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