There are numerous reports of GFP detection by flow; here are a subset Lybarger L, 1996 Rapid generation and flow cytometric analysis of stable GFP-expressing cells Cytometry 25, 211-220 (1996) Zhang G, 1996 An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 227, 707-711 (1996) Anderson MT, 1996 Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 93, 8508-8511 (1996) Ropp JD, 1996 Aequorea green fluorescent protein: simultaneous analysis of wild-type and blue-fluorescing mutant by flowcytometry. Cytometry 24, 284-288 (1996) Zolotukhin S, 1996 A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells. J. Virol. 70, 4646-4654 (1996) Before posting questions how about a Medline search yourself, and trying to ask questions that will stimulate an informative response. That bitchiness aside, I am happy to try to answer more specific questions about GFP detection by flow. Rachel Gerstein UMASS Med. Ctr. Worcester,MA.
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