Hi: I was curious if people had been successfully analysed GFP levels within transfected cells using flow? I once had an investigator try something like this (though I do *not* know if it was GFP, per se) and the experiment was a complete bust! There was something about the excitation/emmision that was not near instantaneous and there was enough delay that no fluoresence could be detected except when using a flourescent microscope. David
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