At 04:06 PM 14/02/97 -0400, Barry Grimes wrote: >Hello all, >I'm planning on doing sterile sorting and will be using ETOH to purge my >FACSVantage. To avoid repeated filling and rinsing steps using only one >tank, I would like to use an extra sheath tank for the alcohol rinse - but >would rather not spend the $1200 for a spare 10 L tank. Is there anyone out >there that might have a spare FACStar / Plus 2 L sheath tank they'd be >willing to sell? Does anyone know if this will in fact work? Yes it will certainly work, I've been doing it for years. I use the waste tank for ETOH, and still use a transparent container for waste. (easy to see if it is about to overflow into the house vacuum line...) Both tanks are online and pressurised, and I use a 3-way lauer-fit valve to select 70% ETOH or saline. NOTE: Be very careful not to run the ETOH into the sheath tank, as the saline will precipitate! I also add an extra 1" 0.22u syringe filter into the line immediately after running ETOH, which has several effects: - Extra filtering of the saline, just in case. - The small filter-full of air stops the saline & ETOH from mixing in the line, so salt precipitation is not a problem. - One minus, it changes the pressure balance so that the sample differential must be reduced by about half with extra filter in. I got this idea from Frank Battye of WEHI, with much appreciation! Frank - you may have some comments; are you still using clear filter holders as fluid reservoirs? Cheers, Joseph. -- Joseph Webster Flow Cytometry Facility Centenary Institute
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