I haven't been so lucky with the luer valves. They tended to
leak. For sorting bacteria I have actually put two 0.22um
disposable filtercartrige (Millipore Millex-GV) parallel
into the sheath line close to the flow cell which is
replaced on the day. Only the line behind those filters is
rinsed with 50ml bleach solution followed by 50ml 75%
isopropanol. This is sucked in by running the flow cell on
vacuum on the Coulter Elite.
As mentioned recently, just make sure the high voltage is
switched off unless you have exceptional insurance cover.
Regards
Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: Re: Wanted: extra BD sheath tank
Author: J.Webster@centenary.usyd.edu.au at INTERNET
Date: 18/02/97 02:13
At 04:06 PM 14/02/97 -0400, Barry Grimes wrote:
>Hello all,
>I'm planning on doing sterile sorting and will be using ETOH to purge my
>FACSVantage. To avoid repeated filling and rinsing steps using only one
>tank, I would like to use an extra sheath tank for the alcohol rinse - but
>would rather not spend the $1200 for a spare 10 L tank. Is there anyone out
>there that might have a spare FACStar / Plus 2 L sheath tank they'd be
>willing to sell? Does anyone know if this will in fact work?
Yes it will certainly work, I've been doing it for years.
I use the waste tank for ETOH, and still use a transparent container
for waste.
(easy to see if it is about to overflow into the house vacuum line...)
Both tanks are online and pressurised, and I use a 3-way lauer-fit
valve to select 70% ETOH or saline.
NOTE:
Be very careful not to run the ETOH into the sheath tank, as the saline
will precipitate!
I also add an extra 1" 0.22u syringe filter into the line immediately
after running ETOH, which has several effects:
- Extra filtering of the saline, just in case.
- The small filter-full of air stops the saline & ETOH from mixing
in the line, so salt precipitation is not a problem.
- One minus, it changes the pressure balance so that the sample
differential must be reduced by about half with extra filter in.
I got this idea from Frank Battye of WEHI, with much appreciation!
Frank - you may have some comments; are you still using clear filter
holders as fluid reservoirs?
Cheers, Joseph.
--
Joseph Webster
Flow Cytometry Facility
Centenary Institute
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