At 03:39 PM 13/02/97 -0800, Karel Drbal wrote: >I ask you whether anyone have recorded a strange laser failure (Ar-15mW, >air-cooled) in the BD instrument (FACSort). It happens almost everyday during >data acquisition. The laser power and current drops to zero and after approx. 1 >minute come back to the normal value. Normal values for laser power and current >were OK. Nothing I have tested helps. The laser have 5000 hours which is close >to its lifetime. >My questions: >1) Could overheating be the cause? Yes, it could. Is the airflow obstructed? Dirty filter? Fan(s) not working? Hot air exhaust restricted? Does it go off repeatedly, or only once per day? >2) How one can decide when the laser should be replaced? I've never seen one go like yours; usually the current increases slowly during the life of the tube, then one day it refuses to work.(ever again) >Any other suggestions? What does BD suggest? What does the laser manufacturer suggest? If a component is failing due to heat, it is likely to be some small electronic bit rather than the laser tube. Cheers, Joseph. -- Joseph Webster Flow Cytometry Facility Centenary Institute
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