Dear flowers, I ask you whether anyone have recorded a strange laser failure (Ar-15mW, air-cooled) in the BD instrument (FACSort). It happens almost everyday during data acquisition. The laser power and current drops to zero and after approx. 1 minute come back to the normal value. Normal values for laser power and current were OK. Nothing I have tested helps. The laser have 5000 hours which is close to its lifetime. My questions: 1) Could overheating be the cause? 2) How one can decide when the laser should be replaced? Any other suggestions? -- _______________________________________________________________ Karel Drbal Laboratory of Leukocyte Antigens Institute of Molecular Genetics Academy of Sciences of the Czech Republic Videnska 1083 142 20 PRAHA 4 Czech Republic, Europe voice: +42-2-4752589 fax: +42-2-4727979 e-mail: drbal@biomed.cas.cz WWW: http://leuko.biomed.cas.cz
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