Small beads staying in the instrument can be a problem. Triton X-100 at 0.1% (the same concentration used to permeabilize cells) in water will remove most of them. Betsy Robertson ========================================================= Betsy R. Robertson (907)474-7709 - Voice Institute of Marine Science 474-7204 - FAX University of Alaska Fairbanks Fairbanks, Alaska 99775-1080 brrob@ims.alaska.edu ========================================================= On Fri, 7 Feb 1997, Antony Bakke wrote: > > Nona Sheila R. Agawin asked: > " is it always very hard to clean the flow cytometer after passing samples with > fluorescent beads ?" > > I have found that small beads (< 4 micron) can stay in the instrument for a long > time appearing in multiple samples after the beads were run. Running ethanol > after the beads seems to help push them through more quickly. > > Tony Bakke > > >
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