>Small beads staying in the instrument can be a problem. Triton X-100 at >0.1% (the same concentration used to permeabilize cells) in water will >remove most of them. > >Betsy Robertson >> >> Running ethanol >> after the beads seems to help push them through more quickly. >> >> Tony Bakke I was wondering about what types of solutions can be run through a flow cytometer without causing damage or corrosion. Is pure ethanol okay? On the advise of others, I have run 50% hot bleach to clear up clogs, but I was very weary about doing this since bleach corrodes stainless steel. I've always followed with a 5 minute water rinse, but this concentration of bleach seems a bit extreme (we routinely use 10% bleach at room temp). If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than this seems to be the way to go. Any thoughts about this? What do manufacturers recommend? Jim -- James A. Zanghi Dept. of Chemical Engineering Northwestern University Evanston, IL
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:22 EST