Dear flowers! We want to analyze marine bacterial populations using flowcytometry. Because we are new in this field, there are a lot of problems. One problem occurs, when putting the PMs to about 500 (which is 50% of maximum - and necessary! for stained bacteria) we get (more or less) huge clouds of signals even with (particle-free) 0.2 µ filtered A. dest. Shall we filter the sheath-fluid again (we use BD sheath fluid). Is it electronic noise? Does anybody have an idea? Gunnar Gerdts Biologische Anstalt Helgoland Dept. Marine Microbiology 27483 Helgoland Germany
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