Dear Flow Cytometrists: Problem Statement: In wanting to determine the minimally infective dose (oral route in a neonatal mouse model as well as in a human colon tumor cell line) of cryptosporidium oocysts, we must obtain highly accurately sorted and calibrated pure cyst suspensions from crude density gradient preps. Main contaminants in crude are yeasts. We have a highly specififc FITC-MAB, fluor tagging of cysts has been well worked out. We have a Coulter Epics Elite equipped with the SPE fast sorter and autoclone, we are experienced sorters. ( We also have a Meridian ULTIMA confocal scope as an adjunct tool) My inquiry is about: 1. Does anyone have experience with flow sorting and/or confocal mapping of protozoan oocysts, about potential problems and technical tricks, etc? 2. Would FITC-MAB tagging and flow sorting of oocysts have an adverse impact on cyst viability, infectivity and virulence? 3. Do I need to worry about aerosol-borne infectious control. Any advice or dialogue will be greatly appreciated. J. Peter Bercz USEPA/NERL/EERD Cincinnati
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:20 EST