Check out Alexis Biochemicals in San Diego-1- 800-900-0065 phone, 1-800-900-9224 fax. On page 211 of their most recent catalog they have monoclonal A11 which is a rat IgM (beware of cytotoxicity) which is purified, biotinylated, or fitc conjugated. All about $490.00. They also list monoclonal H11 which is purified (100ug) and is a rat IgG2a. I am personnally interested in hearing from anyone who has used the IgM conjugates for flow cytometry and sorting. How cytotoxic is it and how bright are the conjugates? The only analysis I have seen has been with polyclonal antibody indirect reagents which were quite adequate but I am looking to use it in multicolor systems. I was just talking with Brian at Alexis- Technical person- he gave me two references for the conjugate clone- Science vol274 Nov22,1996 (Hahne et al) and Int. Immunology 7, 1381 (1995) Hahne et al. Hope this helps, Rochelle Diamond California Institute of Technology Biology Division Flow Cytometry/Cell Sorting Facility diamond@cco.caltech.edu On Wed, 29 Jan 1997, O. Joseph Trask, Jr. wrote: > > Greetings- > Does anybody out there know where I can find mouse CD95L Fas ligand? > Thanks in advance. > Regards, > O. Joseph Trask, Jr. > The Ohio State University > Analytical Cytometry Laboratory > 416 Comprehensive Cancer Center > 410 West 12th Avenue > Columbus, Ohio 43210 > Tel: 614/292-FLOW(3569) > Fax: 614/292-7335 > E-Mail: cytometry@osu.edu > >
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