Dear Flow-ers, My colleague and I would like to set up a test for the expression of cytoplasmic IgM for differential diagnosis of B-ALL and would appreciate any comments /advices /references on the problems that we encountered, mainly how would one tell the difference between a surface IgM versus a cytoplasmic IgM? Can I somehow block the staining of surface IgM or can I quench the surface staining as is being done with phagocytosis tests? And is there any good controls for the tests? We are also evaluating the possibility of performing a cytoplamic CD3 staining for diagnosis of T-ALL and, quiet possibly use it for monitoring of minimal residual disease. We have the same concerns for this test as we mentioned for cytoplasmic c-mu and would appreciate any comments /advices /references. Warm regards, Austin Lin Core Facility Laboratory-Flow Cytometer, Cheng Gung Medical College Tainan, Taiwan
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