Marcl, I have used both commercial hemaline and water as sheath in the FACSCalibur and FACScan with no problems - sometimes I like to clear the lines and the droplet containment motor of salts and run with something a lot cheaper - however some samples are uniquely susceptible to small changes in tonicity as occurs when the drop left behind in the sip tube between samples enters a subsequent sample during sample change. Our Red cell samples for example had to be run exclusively in hemaline and for DNA analysis you may wish to use isoton(hemaline) exclusively because of the noted susceptibility of PI staining to small tonicity differences. For the majority of immunophenotyping however, I would think no problems. Gene Pizzo/UCONN Health
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