Re: negative control for flow crossmatch

From: austin (austin63@ms12.hinet.net)
Date: Tue Dec 13 1994 - 04:03:51 EST


Dear flow-ers,

One of my colleague would like to set up T-cell crossmatching protocol in 
our Transplantation Center. Our first trial have been satisfactory. We, 
however, is not certain about where we can obtain good negative control 
serum for the test. According to some of the reference we have reviewed, we 
basically have to pool serum from at least 20 healthy AB blood type persons 
and use it as a negative control.  The problem is that we do not run T-cell 
crossmatching test very often (approx. 2-3 test/months).  Is there a good 
commercial source for the control serum that we can buy in a smaller 
quantity? Or can anyone give us suggestion on a good alternative source? 
Thank you in advance.


Austin Lin
austin63@ms12.hinet.net
Cheng Gung Medical College,



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