>Does anyone know of a good HRP-conjugated anti-human IgG secondary >reagent? -- James W. Jacobberger, Ph.D. Associate Professor of Oncology >Case Western Reserver University Cleveland, OH 44106-1944 An excellent source for anti-huIgG (both, Fc- as well as Fab'-specific) antibodies conjugated to a variety of detection-molecules including HRP, is Jackson ImmunoResearch; I've been using their antibodies for ELISPOT assays, Western Blot and Immunohistochemistry and I am very content with these conjugates. Good luck!
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