DNA + surface staining

From: Steve Neben (SNeben@genetics.com)
Date: Wed Jan 08 1997 - 12:24:08 EST

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Dear Flow-ers:

I'm looking for a realiable method of staining bone marrow cells for
simultaneous cell cycle analysis and 3-color surface
immunofluorescence. Antibodies are FITC-, PE-, and biotin-conjugated.
I'm using a FACStarPlus with Argon, He-Ne, and UV lasers. Can anyone
suggest a good DNA stain and avidin conjugate that will work for this
configuration ?

Steve Neben
Genetics Institute
Cambridge, MA

Next message: Conley, Christopher R., M.D.: "Re: Lymphomas, Morphology vs. Immunophenotype"
Previous message: Maryalice Stetler-Stevenson: "Clinical Flow Course"
Next in thread: Howard Shapiro: "Re: DNA + surface staining"
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