We are hoping to demonstrate the physical interaction of two cellular proteins using FRET-Fluorescence Resonance Energy Transfer. FRET basically works on the same principles as commercially available PE/Cy5 tandems. If two fluorochromes are in physical proximity and the donor emission and receptor excitation spectra match, you get a non-radiative energy transfer from donor to acceptor molecule with emission at a wavelength characteristic of the acceptor molecule The farther the molecules are away, the less transfer you get. At any rate, I was hoping to do this with a 488nm argon laser with PE as the donor and either Cy5 or APC as the acceptor. Does anyone have any technical gems to share either with this pair of dyes or in general? Thanks. _______________________ Calman Prussin Laboratory of Allergic Diseases NIAID/ National Institutes of Health calman@nih.gov
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