Purified OKT3 is available from several sources. Ortho Diagnostic Systems in Raritan NJ sells OKT3 for flow cytometry purposes. They used to sell it unconjugated, but I think it's been discontinued. However, if you contact them they may sell you some purified antibody with no carrier protein and no preservatives as a special order. If you have contacts with a hospital performing organ transplants you may be able to obtain the therapeutic version of OKT3 (purified antibody, no preservative, no carrier protein) sold by Ortho Biotech from the hospital pharmacy. Finally, the cell line can be purchased from ATCC if you will not be using the antibody commercially or for development of a commercial product and you can grow it up and purify some yourself. Pat Rao >>> Meenakshi Roy <meena@macsun.stanford.edu> 01/04/97 08:32pm >>> Hi, Does anyone know where I can buy purified OKT3? There are many other anti-human CD3 antibodies on the market, but I am going to use this antibody to induce apoptosis. I dont know whether any of the other antibodies have been used to induce apoptosis I would appreciate any info, advice etc, Dr. Meenakshi Roy Stanford University.
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