Hi: I apologize in advance for this message since I realize that my request is only distantly related to flow cytometry... I am trying to find a lab with expertise in fluorescence in-situ hybridization of probes to metaphase chromosome spreads in mammals. I am particularly interested in labs that have done it with dog cells, but as it seems, my probes work in mice and humans also. I would like to physically map my probes to a specific dog chromosome by using chromosome specific reference probes. Has anyone on this list done this or knows a lab that has done FISH extensively? I'd be glad for any hints! Thanks Michael
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