Diatec AS
Gaustadalléen 21
N-0349 Oslo, Norway

Tel: (+47) 22 95 86 25
Fax: (+47) 22 95 86 49
diatec@diatec.com

 select a product

Anti-Human CD14

Product number: Product description: Unit: Price:
3110 unconjugated 0.2 mg/200 µL USD 120, NOK 900
3111 FITC conjugated 100 tests/2 mL USD 135, NOK 1050
3112 PE conjugated 100 tests/2 mL USD 150, NOK 1125

Isotype: mouse monoclonal IgG1
Clone: 18D11
Production: In Vitro hollow fibre production system
Purification: Protein A
FITC conjugate F/P-ratio: 7.1 (B157)

transportation costs, customs and local tax will be added to our net prices.
This product is for in vitro research use only, and not for household, diagnostic or in vivo use.


Specificity
This antibody recognises the CD14 antigen (LPS receptor) expressed strongly on the surface of monocytes, weakly on the surface of granulocytes, macrophages, dendritic cells and B-cells. On flow cytometry it stains >90% of human peripheral blood monocytes.
The antibody is LPS neutralising.

Performance
Flow Cytometry:
Recommended use of FITC, PE and APC-conjugates:
Whole blood: 100mL blood + 20 mL mAb
Cell preparations: 50mL (500 000) cells + 20 mL mAb
Wash is recommended to yield optimal results. For more details, see Flow cytometry methods page

FITC conjugate
CD14 FITC

Example data: Whole blood gated on monocytes

Diatec Clone (red)
Anti-human CD14 FITC (subclass IgG1)
Product number P3111, Batch no 157
Concentration: 16 µg/mL

Competitor mAb (black)

Isotypic control (Blue) IgG1 FITC.
Product number P2011, Batch no 054
Concentration: 50 µg/mL


PE conjugate
CD14 PE

Example data: Mononuclear cells gated on lymphocytes

Diatec Clone (red)
Anti-human CD14 PE (subclass IgG1)
Product number P3112, Batch no 154
Concentration: 6.25 µg/mL

Becton Dickinson (black)
anti-human CD14 PE
Batch no 71132

Isotypic control (Blue) IgG1 PE.
Product number P2012, Batch no 077
Concentration: 50 µg/mL

Storage
Unconjugated antibody: The antibody is diluted in phosphate buffered saline, pH 7.4.
For long term storage, -20ºC is recommended, but it may be stored for short term use at 4ºC. The expiry date on the vial refers to storage at -20ºC.
Repeated freeze/thawing is not recommended.

Conjugated antibody: The antibody is diluted in phosphate buffered saline, pH 7.4 containing 0.2% BSA.
Storage at 4ºC. The expiry date on the vial refers to storage at 4ºC.

Preservative
Sodium azide is used as a preservative at a concentration of 0.1%. Sodium azide under acid conditions yields hydrazoic acid, an extremely toxic compound.

Antigen Structure and function 1
CD14 is a GPI-linked glycoprotein.
CD14 is a receptor for LPS (Lipopolysaccaride complex) and for LPS-binding protein (LBS).

Antigen tissue distribution 1
The CD14 antigen is expressed mainly on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. It is also expressed at low levels on neuthrophils and on human B-cells.

References

  1. Barclay, Brown et al., The Leucocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997)
Back to top